Hampden Park has missed out on hosting the opening match of the 2020 European Championships.
UEFA has announced the Stadio Olimpico in Rome will be the venue for the showpiece match for the 60th anniversary championships, which will be played across the continent.
The Italian stadium has beaten off competition from Hampden, the Krestovsky Stadium in St Petersburg and the Johan Cruyff Arena in Amsterdam, which were all vying to host the opening game.
The Eurostadium in Brussels was also in contention but will no longer take part in hosting due to delays to the new purpose-built stadium.
Wembley, which was already scheduled to host the semi-finals and final, will now replace the stadium and stage three group games and one last 16 match.
England's national stadium has been paired with Glasgow to host the six matches in Group D.
The tournament will be played across Europe for the first time in 2020 to commemorate its 60th anniversary, with European football's governing body choosing Hampden as one of 13 host venues.
The Glasgow ground is already guaranteed to have three games from the group stages and one from the round of 16.
With a capacity of 52,025, the ground has hosted the UEFA Cup final in 2007 and the Champions League final in 2002 since its redevelopment.